| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Osteoprotegerin, a new actor in vasculogenesis, stimulates endothelial colony-forming cells properties
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| Autores: |
Benslimane-ahmim, Z.
Heymann, D.
Dizier, B.
Lokajczyk, A.
Brion, R.
Laurendeau, I.
Bieche, I.
Smadja, D. M.
Galy-fauroux, I.
Colliec-jouault, Sylvia
Fischer, Anne-marie
Boisson-vidal, Catherine
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| Data: |
2011-04
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| Ano: |
2011
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| Palavras-chave: |
Endothelial colony-forming cells
Osteoprotegerin
Receptor activator of nuclear factor-kappa B ligand
Vasculogenesis
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| Resumo: |
Background: Osteoprotegerin (OPG), a soluble receptor of the tumour necrosis factor family, and its ligand, the receptor activator of nuclear factor-kappa B ligand (RANKL), are emerging as important regulators of vascular pathophysiology. Objectives: We evaluated their effects on vasculogenesis induced by endothelial colony-forming cells (ECFC) and on neovessel formation in vivo. Methods: Effects of OPG and RANKL on in vitro angiogenesis were evaluated after ECFC incubation with OPG or RANKL (0-50 ng mL(-1)). Effects on microvessel formation were evaluated with an in vivo murin Matrigel plug assay. Vascularization was evaluated by measuring plug hemoglobin and vascular endothelial growth factor (VEGF)-R2 content 14 days after implantation. Results: We found that ECFC expressed OPG and RANK but not RANKL mRNA. Treatment of ECFC with VEGF or stromal cell-derived factor-1 (SDF-1) upregulated OPG mRNA expression. OPG stimulated ECFC migration (P < 0.05), chemotaxis (P < 0.05) and vascular cord formation on Matrigel (R) (P < 0.01). These effects were correlated with SDF-1 mRNA overexpression, which was 30-fold higher after 4 h of OPG stimulation (P < 0.01). OPG-mediated angiogenesis involved the MAPK signaling pathway as well as Akt or mTOR cascades. RANKL also showed pro-vasculogenic effects in vitro. OPG combined with FGF-2 promoted neovessel formation in vivo, whereas RANKL had no effect. Conclusions: OPG induces ECFC activation and is a positive regulator of microvessel formation in vivo. Our results suggest that the OPG/RANK/RANKL axis may be involved in vasculogenesis and strongly support a modulatory role in tissue revascularization.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00033/14462/11863.pdf
DOI:10.1111/j.1538-7836.2011.04207.x
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| Editor: |
Wiley-blackwell
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| Relação: |
http://archimer.ifremer.fr/doc/00033/14462/
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| Formato: |
application/pdf
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| Fonte: |
Journal Of Thrombosis And Haemostasis (1538-7933) (Wiley-blackwell), 2011-04 , Vol. 9 , N. 4 , P. 834-843
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| Direitos: |
2011 International Society on Thrombosis and Haemostasis, Wiley-Blackwell
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